There are two main types of multiplexed ELISA assays that are commonly used: sandwich and competitive.
Sandwich ELISAs are the most widely used type of assay and involve the capture of an analyte by one antibody, followed by the detection of the analyte with a second antibody. This type of assay is highly specific and sensitive, making it ideal for a variety of applications. Look at this site for more information about Multiplexed ELISA Assays.
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Competitive ELISAs are less common but can be useful in situations where the analyte concentration is very low or when there is a need for increased specificity. In this type of assay, the capture antibody and detection antibody are both directed against the analyte. The capture antibody competes with the analyte for binding to the detection antibody, resulting in a lower signal.
Both types of assays can be multiplexed, allowing for the simultaneous detection of multiple analytes in a single sample.
Advantages of Multiplexed ELISA Assays
There are several advantages to using multiplexed ELISA assays:
1. Increased throughput: By being able to detect multiple analytes in a single sample, multiplexed ELISAs can significantly increase the throughput of a laboratory.
2. Reduced costs: Multiplexed ELISAs require less reagents and labor compared to traditional ELISAs, which can lead to significant cost savings.
3. Increased sensitivity: The simultaneous detection of multiple analytes can increase the overall sensitivity of the assay.
4. Increased specificity: The increased specificity of multiplexed ELISAs can allow for the detection of low-abundance analytes that would be undetectable in a traditional ELISA.